Characterization of diarrhoegenic escherichia coli using a novel multiplex PCR
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چکیده
منابع مشابه
Rapid Diagnosis and Characterization of Diarrheagenic Escherichia coli In Egyptian Children Using Multiplex PCR
Escherichia coli (E.coli) is the most important etiologic agent of childhood diarrhea and represents a major public health problem in developing countries. Aim of this work was to investigate the role of diarrheagenic E.coli in Egyptian children below 5 years age using multiplex PCR and to evaluate multiplex PCR in rapid diagnosis of enteric infections caused by diarrheagenic E.coli strains. Re...
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A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli s...
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BACKGROUND Diarrheagenic E.coli (DEC) are an important cause of childhood diarrhea.Identification of DEC strains needs to detect factors that determine the virulence of these organisms. There is not much data regarding the importance of DEC as a cause of diarrhea in children in India.The prevalence of DEC in children below five years with and without diarrhea was studied using two multiplex PCR...
متن کاملIdentification of Biofilm Encoding Genes (agg) in the Escherichia coli Isolates by Multiplex-PCR Method
Introduction: Biofilm is a collection of microbial cells that are irreversibly suppressed and mildly washed away. The aggR gene is located on the main plasmid (pAA), which codes for many actuator factors. The pathogen strains of EAEC have an aggR gene, the gene has been identified in six classes of pathogenic E. coli. Materials & Methods: In the present study, 60 stool samples from children ...
متن کاملIdentification of Different Categories of Diarrheagenic Escherichia coli in Stool Samples by Using Multiplex PCR Technique
Diarrheal diseases continue to be one of the most common causes of morbidity and mortality among young children in developing countries. The objective of this s tudy was to evaluate the multiplex PCR as a rapid diagnostic tool for simultaneous detection of four categories of diarrheagenic E. coli (ETEC, EPEC, EHEC and EAEC) in one PCR reaction using six virulent genes. During the period from Ju...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2016
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2016.02.345